The game begins with a cutscene showing Rayman having a peaceful picnic with the Baby Globoxes until it is interrupted by a brief earthquake that causes the Globox babies to be submerged into the ground. Suddenly, three large Rabbids emerge from the ground. Rayman offers them food, but is instead kidnapped by Serguei, a large gorilla-like rabbid. Soon Rayman is thrown into a colisseum surrounded by thousands of Rabbids. Rayman is then put through a series of trials and competitions, earning plungers by winning them. Rayman is put in a cell and realizes that he can build a ladder with the plungers in order to escape through the window. As the game progresses, the Rabbids begin to cheer on Rayman, thus making him more popular among the Rabbid community. At the end of the game, Rayman manages to escape through the window, but soon finds out that the Rabbids did not force him to do the mini-games just for amusement, but rather to teach them how to do it so they can do the same to the Baby Globoxes. He tries to go through the same hole the globox kids were sunk into, but instead gets stuck in the hole himself. It is unknown how the Rabbids react to Rayman's disappearance nor the fate of the Globox babies. While the console versions and DS version have the same plot and are almost identical, there are some differences. Both involve Rayman getting captured by the Rabbids, taken to the colisseum and Rayman has to escape, but in the DS version Rayman has to get 100 trophies and defeat the Rabbid droid, while the console versions only have Rayman get 15 plungers in order to escape
Rayman - The main protagonist the Rabbids have captured along with the Baby Globoxes. Rayman is obviously much more intelligent, cunning, and powerful than the Rabbids and manages to outsmart them in most of the mini-games they put him through.
Baby Globoxes- The deuteragonists. The cute little babies are also the ones kidnapped along with Rayman and are later revealed to be the true intended victims of the Rabbids' mini-games as Rayman was just a practice for them.
Rabbids - The main antagonists. The wild, chaotic, and seemingly brainless rabbit-like creatures that kidnap Rayman and the Globox Babies to put them through a series of mini-games for their entertainment.
Serguei - A large, menacing, and ape-like Rabbid that is in charge of guarding Rayman while he is kidnapped.
Trolls - Stone-like monsters that occasionally appear in mini-games to help the Rabbids.
- This is the first and last time we see Serguei it is unknown if he returns in Rabbids Invasion. *The gba version and the DS version are both platformers/party but they are different to each other. The gba version is closer to that of the beta version. The DS version is the same soundtrack as the console versions and features minigames that many are based on the console versions. The minigames of the DS version can also be unlocked in extra mode and the score as many points as you can. In some minigames of the console versions and the DS version the rabbis are playable.
- The handheld versions are not only platformers but also are party games. All versions are party games.